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RET/PLT-O Channel

In the RET/PLT-O channel, the surfactant in RET-SEARCH (II) slightly perforates the cell membranes of red blood cells, white blood cells and platelets and so allows the fluorescence marker to penetrate the cell. The fluorescence marker then labels the nucleic acids in white blood cells, NRBC, reticulocytes and platelets. Using the forward scattered light and the fluorescence signal, the reticulocytes can be separated from mature red blood cells, white blood cells and NRBCs.

According to their fluorescence intensity, reticulocytes are fractionated into three categories, representing different stages of maturity:

  • LFR (low fluorescence reticulocytes)
  • MFR (medium fluorescence reticulocytes)
  • HFR (high fluorescence reticulocytes)


The IRF (immature reticulocyte fraction) reflects the proportion of immature reticulocytes and is calculated from the sum of MFR plus HFR.

The RET channel also provides the optical platelet count and is available on the XT-2000i, XT-4000i, XE-2100 and XE-5000 haematology analysers.

Analyser channels

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