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In this maturation stage the separation into the 3 different subpopulations of granulocytes occurs by development of specific granulation for each (secondary granulation).

Cell description:

Size: 10-18 µm

Nucleus: oval or slightly indented with variable degree of chromatin clumping, nucleoli usually not apparent

Cytoplasm: acidophilic neutrophil: primary azurophilic and secondary neutrophilic granules

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