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Blood Film Master Advanced

  • Standardised staining of blood smears
  • Ideal for manual differential and automated digital morphology
  • Simple to operate
  • Automated

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From smear production to smear staining

The Blood Film Master Advanced standardises and optimises the manual blood smear preparation by using the RAL Stainer and MCHD Reagents.

Blood film preparation and staining are well known techniques in haematology laboratories. In principle it is a very simple procedure but there are many reasons why the test can easily fail or be less effective than it should be. Most haematology laboratories use the wedge technique for preparation of blood smears and a manual method or automated slide stainer to stain the prepared blood smears.

The Blood Film Master Advanced provides a better quality and standardisation of the staining of blood smears which leads to a better judgement of the blood cell morphology.

 

 

RAL Stainer

RAL Stainer

Once you have produced a great blood film, you want a great stain. If you want to ensure you produce consistently high quality levels and standardisation, we recommend the RAL Stainer. It is a compact, simple, intelligent and safe automated staining system for staining whole blood smears that is used in combination with our methanol-free MCDh staining reagents. It also reduces staining time by almost half compared to standard methods.   

Fast staining

One of the reasons the stainer is so fast is that you can load up to 10 slides into a slide holder, which is then placed in one of the two loading drawers. Press the start button and the slide holder is dipped in each of the 5 staining stations. The standard MCDh staining protocol has been optimised for peripheral blood smears, which are stained within 15 minutes. Because it has two loading drawers, loading and unloading is continuous. And while the staining process is going on, your technicians can confidently get on with other tasks.

Maintenance-free and safe

One of the major time-consumers in staining is maintenance. You will be glad to hear the stainer needs none. And your staff are entirely safe as well, as the staining is performed in a completely closed system – an active carbon filter neutralises reagent vapours.

RAL Stainer - Key Benefits

RAL Stainer - Key Benefits

Key benefits

Simple:

Ready to use MCDh reagents Kit for direct loading

No priming of staining reagents required

Customisable staining protocols

 

Safe:

Reliable and robust mechanism

No hazardous exposure

No hazardous handling

Activated carbon filter neutralises reagent vapours

Automatic draining of expired reagents

 

Intelligent:

RFID (Radio Frequency IDentification):

  • Automatic detection and management of reagents kit
  • Traceability of stain cycle performance
  • Detects rinsing and effluent liquid levels

 

Efficient:

Low reagent consumption

No routine maintenance needed

Standardised and repeatable staining protocols

MCDh Reagents

MCDh Reagents

MCDh (Micro Chromatic Detection haematology) is a panoptic haematology staining method. It is methanol-free and consists of a sequence of four specific reagents for staining peripheral blood smears in the haematology laboratory.

The chemical properties of each reagent gradually reveal blood during the staining process. So does the appearance of a purple coloration (Romanowsky-Giemsa type effect), notably on chromatin, platelets and neutrophilic granules.

The reagents deliver superb stains in line with standardisation trends in diagnostics techniques. Importantly, the staining time is almost 50% shorter compared to the standard May Grünwald – Giemsa staining method for manual blood film staining. This means you can save significant amounts of time, especially if you have larger volumes of stains.

Using the RAL stainer, the four reagents are used one after the other, and always in the correct order. The MCDh reagents deliver stains of standardised, reproducible quality. This is further enhanced by the limited number of manual processes. It sets the stage perfectly for the next step – digital morphology.

MCDh Reagents – Stain Components

MCDh Reagents – Stain Components

The MCDh reagents kit consists of four components that are matched in terms of volume and composition.

The process is much simpler than regular procedures as there are far fewer steps involved. This has a knock-on effect as it reduces documentation (SOPs, QMS manual) and delivers smoother workflows that are easier to follow for staff and QMS auditors alike.

Thanks to fewer manual interventions, you reduce potential errors and produce stains of uniform staining quality. As a result, you will find it easier to compare differentiation results.

Staining mechanism

MCDh 1

This is a blend of neutral stains used to fix smears and to prepare staining mainly of water-soluble elements (basophilic granules). These stains are not active in alcohol media and only act selectively when they are released into buffered aqueous solution.

MCDh 2

This reagent leads to concentration and precipitation of the stain on the erythrocytes, neutrophilic granular of the cytoplasm and on eosinophilic granules.

MCDh 3

Is used for staining the monocyte and lymphocyte cytoplasm, and performs the red metachromatic staining of azure granules.

MCDh 4

Is a rinse that removes excess stain from the cells and finalises the staining.

Specifications

Specifications

Throughput

Depending on the used staining protocol.

  • Standard stain protocol: 11 min
  • Drying cycle: 5 minutes
  • Staining baths    4 staining and 1 rinsing bath
  • Loading capacity    Up to 10 slides per basket
  • 2 loading stations

MCDh Reagents

  • MCDh reagent Kit Ready to use on RAL Stainer
  • Stain capacity Maximum 500 slides
  • Shelf Life 18 months unopened
  • 21 days after opening
  • Storage Temperature 15 to 30°C
Dimensions and Weight

Dimensions and Weight

Dimensions: 62.4 (H) cm x 42.5 cm (D)

Weight: 28 kg

Ambient temperature: 15 to 30°C

Power Consumption: 150 VA or less, 50 – 60 Hz, 110 – 230 V

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