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CyFlow Ploidy Analyser

  • Screening for Haplo-, Diplo-, Triplo- and Polyploids
  • Ploidy and Genome size analysis in less than two minutes
  • Detection of Anisoploids, Allopolyploids and Aneuploids
  • No time consuming microscopic evaluation
  • Any plant material can be analysed: leaves, seedlings, roots, flowers, peel of fruits, seeds

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Automatic Measurement of Ploidy Level and Genome Size

High-performance flow cytometry

The CyFlow Ploidy Analyser is a compact flow cytometer for ploidy analysis, high resolution DNA and genome size analysis for plants, animals and micro- organisms.

This modular system is equipped with a UV LED (365nm) and/or a green laser ( 532nm) which, when used in combination with Partec CyStain reagents, provides high resolution DNA analysis with DAPI or propidium iodide.

The CyFlow Ploidy instrument measures the DNA content of many thousands of individual nuclei within seconds or minutes. The result is directly displayed as a DNA histogram on the internal 15.4” colour display. The automated, real-time ploidy analysis is extremely time- and cost- efficient.

Flow cytometry
products

Benefits

Benefits

  • Plant Genome Size and C-Value Determination
  • Detection with +/- 1 chromosome accuracy in many plants
  • Detection of Apomixis and Reproduction Behaviour
  • Cytotype Identification in Natural Populations
  • Hybrid Detection
  • Polysomaty and Chimeric Analysis
  • Sex Detection – Nuclear Base Composition
  • Preparation and staining of metaphase chromosomes is not necessary
  • Easy sample preparation by ready-to-use protocols
  • Propidium Iodide and DAPI based DNA fluorescence
  • Early stage tests on seeds and seedlings
  • Portable system for field investigation
Specification

Specification

Light sources:

Up to two light sources

  • UV LED (365 nm)
  • Nd-YAG green laser at 532 nm (30 mW)

Optics:

  • Standard set-up and filters for propidium iodide (long pass filter 590 nm) and/or DAPI/SSC (long pass filter 435 nm)

Flow system:

  • Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
  • True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
  • Completely closed fluidic system
  • Sample port with biosafety cleaning system

Software:

  • Windows™-based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
  • Peak analysis function

Dimensions:

  • Stand-alone instrument: W 385mm x H 290mm x D 280mm
  • With Autoloading Station: W 745mm
  • With screen open: H 528mm
  • Weight: 11.5kg
Software

Software

CyView™: flow cytometry data acquisition and analysis software

  • Windows™-based FCM software CyView™ for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
Upgrades

Upgrades

CyFlow® Robby 6 Autoloading Station

General facts:

  • Protective housing with no light incidence 
  • Automatic washing between samples
  • Start up time of < 5 minutes
  • Seamless easy to use acquisition software

 

Microplates and tubes:

  • 48- or 96-well plates, V-, U- and flat-bottom are fitting (max. two) 
  • Plates meeting the standards ANSI/SLAS (1-2004 through 4-2004) 
  • 2mL-tubes (max. 120)

Flexible choice of sample volume:

  • Up to 180µl for plates
  • Up to 2000µl for tubes

Speed:

  • User definable from 0.2µl/s to 10µl/s

Autoloader dimensions:

  • W 360mm x H 270mm x D 370mm
Applications

Applications

Absolute cell and particle counting

  • Detection of Ploidy Level
  • Genome size
  • DNA analysis
  • Anueploids and Allopolyploids
  • Detection of Apomixis and Reproduction Behaviour
  • Sperm Cell Counting
  • Detection of hybrids
  • Polysomy
  • Chimera Analysis
  • Aquaculture
Complementary products

Complementary products

Sample preparation

  • Celltrics
  • CyStain® UV Ploidy (DAPI)
  • CyStain® UV Precise P (DAPI)
  • CyStain® UV Precise P automate (DAPI)
  • CyStain® UV Precise T (DAPI)
  • CyStain® UV Precise T automate (DAPI)
  • CyStain® PI Absolute P (PI)
  • CyStain® PI Absolute T (PI)

 

 

Contact

Sales representative Sysmex UK

0333 320 3461
sales@sysmex.co.uk

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