CytoCell in Multiple Myeloma
Tri-Colour CytoCell myProbes® for multiple myeloma clinical research
What are the benefits of using three-colour FISH probes?
Enable faster identification of atypical fusions.
Enable analysis of more targets simultaneously.
Enable efficient use and conservation of enriched CD138+ cell samples.
All of the above
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Scientific background
In translational oncology research, optimising the use of limited biological samples is critical, especially when multiple genetic targets must be assessed for comprehensive characterisation. Research institutions are increasingly adopting advanced molecular techniques – such as three-colour fluorescence in situ hybridisation (FISH) probes – to investigate complex genomic alterations efficiently and with minimal sample consumption.
A research sample derived from a physically active adult male donor without prior bone pathology was evaluated following a spontaneous fracture. An imaging investigation revealed extensive osteolytic lesions, leading to the hypothesis of a plasma cell neoplasm, such as multiple myeloma (MM). Given the complexity of differential diagnosis, an initial panel of FISH analyses was performed, including:
- TP53 Deletion
- CKS1B/CDKN2C Amplification/Deletion
- IGH/FGFR3 Breakapart/Dual Fusion
- IGH/CCND1/MAF
The Tri-Colour CytoCell myProbes® IGH/FGFR3 Breakapart/Dual Fusion proved especially informative, revealing a deletion of one IGH allele and a rearrangement of the second – findings that would likely have been missed with conventional two-colour probes. Based on this result, the investigation was then expanded to include:
- IGH/MAFB Dual Fusion
- IGH/MYC Dual Fusion
Further testing identified a 1p deletion and a t(8;14) IGH::MYC / MYC::IGH translocation. These abnormalities are typically excluded from standard panels and would have remained undetected without the broader multiplex strategy.
This case highlights the importance of strategic probe selection and test optimisation in research workflows to uncover clinically relevant genomic events that may otherwise go unnoticed.
Advantages of three-colour FISH probes
CytoCell myProbes® IGH/FGFR3 Breakapart/Dual Fusion Probe V3 (MPD57050)
Probe specification
- IGH, 14q32.33, Red/Green
- FGFR3, 4p16.3, Aqua
The use of three-colour probes in MM diagnostics reduces the number of slides required, which is important in the case of a small number of CD138+ sorted cells. It also facilitates the assessment of unbalanced translocation thanks to the observation of an incomplete IGH gene signal – which, in a low percentage of cells, could be interpreted as a random overlap of signals in the two-colour probe.
CytoCell myProbes® IGH/CCND1/MAF Dual Fusion Probe V2 (MPD22710)
Probe specification
- IGH, 14q32.33, Red
- CCND1, 11q13.3, Green
- MAF, 16q23.1-q23.2, Aqua
The use of the three-colour IGH/CCND1/MAF probe offers additional advantages. By eliminating the gap in MAF gene labelling and covering the CCND1 and MYEOV genes, it allows abnormalities to be detected on a single slide rather than two, and enables detection of t(11;14).
Acknowledgement
Ariel Najdowski
Senior Molecular Genetics Specialist
Department of Molecular and Cell Genetics
Nicolaus Copernicus University in Toruń
Bydgoszcz, Poland
Disclaimer
CytoCell® is a registered trademark of Cytocell Limited. Product availability may vary from country to country and is subject to varying regulatory requirements. Contact your local representatives for availability.
CytoCell myProbes®: For Research Use Only; Not for Use in Diagnostic Procedures.
