Fluorescence Flow Cytometry:
The next step in urine particle analysis

Fast and sensitive urine sediment analysis: rely on thousands of particles counted and classified from native urine. Analysing native urine without centrifugation or pre-treatment avoids common sources of error and makes analysis even more standardised.

Signal detection and particle fingerprints

1. Fluorescence staining

Specific fluorochromes for core (DNA) and surface (membrane) structures underline the characteristics of every particle, increasing the variety of clinical parameters can be determined.

2. Hydrodynamic focusing

A sheath reagent flow separates and aligns the particles in the sample to ensure their individual and proper measurement.

3. Particle characterisation by laser scanning

As each particle passes the 488nm-laser beam, a pattern of individual light signals is generated and captured by a set of detectors.

4. Unique fingerprints

Detected light signal patterns are translated into individual 'fingerprints' allowing their identification and allocation to the according parameter.  Ultimately, this leads to the quantification of each parameter.

5. Scattergram

All signals from one measurement are allocated to the corresponding parameters and summarised in scattergrams. Therefore each scattergram represents the signals of one sample for one parameter.

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